FastQCFastQC Report
Thu 26 May 2016
SRR1781383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21760
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG656630.174632352941178No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG13776.328125No Hit
ATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGGTC5982.7481617647058822Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG3581.6452205882352942No Hit
CTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGG1760.8088235294117648No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT1540.7077205882352942No Hit
CTTTACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGG1300.5974264705882353No Hit
TCTTAATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG1260.5790441176470589No Hit
CTTAATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT1080.49632352941176466No Hit
CTTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT1070.49172794117647056No Hit
TATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGGT810.3722426470588235No Hit
TTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGG610.2803308823529412No Hit
TCTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG400.1838235294117647No Hit
ACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGGTCGC390.1792279411764706Illumina Single End PCR Primer 1 (95% over 23bp)
CTTATACACATCTGACGCTGCCGACGAGCTCATGAGTGTAGATCTCGGTG320.14705882352941177No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG310.14246323529411764No Hit
CTTATATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG300.13786764705882354No Hit
CTTTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG240.11029411764705882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA207.773003E-569.967821
TCTTTAT700.069.967821
CTTTATA800.061.221842
TTTATAC850.057.6205563
AAGGGGG700.054.97471670
TAGGAGT200.00687363652.47586453
ACCATCA200.00687363652.47586419
GATTTTA200.00687363652.47586465
GTCTATG200.00687363652.47586413
AAAGGGG800.048.10287569
TTACACA305.7654723E-446.6452143
TTTACAC305.7654723E-446.6452142
CTTATAC2500.041.980691
TAATTAA350.001217666340.07373446
TTATACA3350.033.417462
TATACAC3500.032.9848253
AAAAAGG2700.031.09680770
AAAAGGG1403.45608E-1027.48735868
CCGTATC4200.025.82145555
TAGATCT4250.025.57647139
Produced by FastQC (version 0.11.5)