FastQCFastQC Report
Thu 26 May 2016
SRR1781383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21760
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG446920.537683823529413No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT8243.786764705882353No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT6212.853860294117647RNA PCR Primer, Index 45 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC4982.2886029411764706No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT4762.1875No Hit
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC3911.7968749999999998No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG1760.8088235294117648No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC1750.8042279411764706No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG1210.5560661764705882No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC1090.5009191176470589No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT940.4319852941176471No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC900.4136029411764706No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT840.3860294117647059No Hit
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT610.2803308823529412No Hit
ATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC460.2113970588235294No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCC440.2022058823529412No Hit
ATTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG410.18841911764705882No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG400.1838235294117647No Hit
ACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCT390.1792279411764706RNA PCR Primer, Index 45 (95% over 23bp)
GTGTATAAGAGACAGCATCTCTTATACACATCTCCGAGCCCACGAGACTC380.1746323529411765No Hit
ATTATAAACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT330.1516544117647059No Hit
AATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT280.12867647058823528No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCG280.12867647058823528No Hit
ATTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC270.12408088235294117No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCAGT250.11488970588235295No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTATTAATCTCGTATGCCGT250.11488970588235295No Hit
TCTCATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG250.11488970588235295No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC240.11029411764705882No Hit
ATAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT240.11029411764705882No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT240.11029411764705882No Hit
TCTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT220.1011029411764706No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAG220.1011029411764706No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATAAATCTCGTATGCCGT220.1011029411764706No Hit
ATTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG220.1011029411764706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC850.070.000011
GATGGCT150.002195593270.015
TTTTGAC150.002195593270.034
ATCTCTT150.002195593270.017
GACAGCA150.002195593270.011
CATCTCT150.002195593270.016
AGCCGAC150.002195593270.045
ACGGCGG150.002195593270.068
AAGCCGA150.002195593270.044
CATATAC207.7553195E-570.02
CAACGAG150.002195593270.020
CTTTACA453.6379788E-1269.999991
CTTTATA850.065.8823552
TCTTTAT800.065.6251
TTTACAC501.2732926E-1163.0000042
TTTATAC850.061.764713
TTACACA553.274181E-1157.2727243
AATCTCC252.3390856E-456.0000049
GCGCCCA252.3390856E-456.00000416
GCCGACA252.3390856E-456.00000446
Produced by FastQC (version 0.11.5)