FastQCFastQC Report
Thu 26 May 2016
SRR1781382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714801
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26238336.70713946958664No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG78761.1018451289239943No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT8330.11653593097939147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG252.3845326E-456.0279634
CGTAGCA252.3853581E-456.0240456
GAATCGT200.00692956852.54094338
GCATCGA200.006941097352.5188663
AACGTAT200.00694879152.5041531
CGTCTTT200.00696805452.4674114
CGCGAGT200.00696805452.4674117
CGCTATT200.00696805452.4674114
ACCGTCG200.00696805452.4674114
ACCATCG200.006969982852.463748
GTCGTAT200.006969982852.4637411
TGAACGG200.006971911552.460075
TGTACGC200.006977700652.4490623
CGTATCA12600.051.96674356
GCCGTAT12850.051.22820754
CCGTATC12650.050.9309555
CGCCGTA12600.050.85515653
GATCTCG12950.050.85043341
TGGTCGC12850.050.67967649
ATCTCGG13050.050.46077742
Produced by FastQC (version 0.11.5)