FastQCFastQC Report
Thu 26 May 2016
SRR1781382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714801
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT15950822.315021943170198No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG290154.0591717135258625No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT168892.362755508176402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121731.7029914619593425No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC95941.3421917428766887No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT37800.5288185103266504No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC37520.5249013361760826No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT26270.3675148747693414No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT19980.27951835545837234Illumina PCR Primer Index 10 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT14540.2034132576759126No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT12880.18019001092611792No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG10700.1496920121824116No Hit
AATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT10490.14675413156948577No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9450.1322046275816626No Hit
ATAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT8160.11415764667368959No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC7580.10604350021894204No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG7480.10464450945088213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAG150.002225150169.99531634
AGGGGGG222950.067.595370
ATTATAC25700.063.3270341
AAGGGGG266350.056.47591469
TCGTAAG3250.055.99233240
TATGCCG273900.054.37736543
CCGTCTT268500.054.37616347
TGCTTGA268150.054.3557955
ATGCCGT273950.054.3291244
GCCGTCT271100.054.31937846
GTATGCC274700.054.28270342
CGTCTTC269700.054.2639848
TGCCGTC273700.054.2508845
CTGCTTG267650.054.23505854
TATCTCG275400.054.22476236
GCTTGAA273650.054.17506856
ATCTCGT277800.054.14305537
CTCGTAT278450.054.1297839
TCTCGTA278000.054.1166938
AAGCCGT3300.054.08350444
Produced by FastQC (version 0.11.5)