FastQCFastQC Report
Thu 26 May 2016
SRR1781381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910897
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35737139.232866064988684No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG124151.362942242646534No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT13030.14304581088750978No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA150.00222056970.0332549
CGCTACG150.00222056970.0332551
CGTGACG150.00222105370.02939646
TATGCGC352.9881448E-759.9625973
TTGTGCG1000.059.4726942
ATCTCGG22900.054.75117542
GATCTCG22900.054.75117541
TCTCGGT22800.054.68409743
TGTGCGC900.054.4105033
CGTATCA22600.054.3782556
TGGTCGC22950.054.0125749
CCGTATC22350.053.89583255
CTCGGTG23400.053.87468744
CGGTGGT24250.053.8576646
TCGGTGG24400.053.81653245
CGTCTGG1950.053.8155369
TCGCCGT22450.053.81173752
GTGGTCG23900.053.76733848
GGTCGCC23000.053.74290550
TGCCGGC2350.053.59241519