FastQCFastQC Report
Thu 26 May 2016
SRR1781381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910897
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT20412322.409010019793676No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG338213.712933514985778No Hit
ATTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT217562.3884149360465563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197152.16435008568477No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC107291.1778499654735937No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC66490.7299398285426344No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT52900.5807462314619545RNA PCR Primer, Index 32 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT52490.5762451737133837No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT32840.36052374747089955No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT25140.27599168731481166No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC24140.2650134976841509No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC23920.2625982959654055No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT21110.23174958310324878No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG18670.2049628004044365No Hit
AATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT14580.16006200481503396No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC14160.15545116517015645No Hit
TCATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG13860.15215770828095823No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC12680.13920344451677852No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT12680.13920344451677852No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC11850.13009154712333007No Hit
ATAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT11680.12822525488611775No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC11140.12229703248556092No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG9830.10791560406939534No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACTTAATCTCGTATGCCGT9650.1059395299358764No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT9640.1058297480395698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA301.024473E-769.9958948
ATACGAG207.930045E-569.9958812
AGGGGGG299050.065.56312670
ATTATAC35300.060.5771681
CGACTAC900.058.32990348
TCGTAAG4550.057.68891540
ATGCCGA6100.057.37367644
CCGTCTA5750.057.21402447
TGCCGAC5750.057.21402445
CACGTAT1850.056.7534239
CCGACTT4900.056.42524747
ACTCGTA5650.056.36836238
AAACTCG6750.055.48126636
ACCGAGC6550.054.5006113
CGAATTC452.7041096E-854.44124248
CTCGAAA900.054.44124239
TCTCGAA4700.054.3585138
AAGGGGG361800.054.08553769
AACTCGA1300.053.8459437
CTACGAG2600.053.84298712
Produced by FastQC (version 0.11.5)