FastQCFastQC Report
Thu 26 May 2016
SRR1781380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455597
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37343881.96673814796848No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG121032.656514419541832No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT12570.2759017289402696No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7050.15474201981136837No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6210.13630467277001385No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5680.12467158475582586No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4860.10667322216783692No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG4850.10645372994115412No Hit
ATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTGGTC4770.1046977921276918Illumina Single End PCR Primer 1 (95% over 21bp)
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4640.1018443931808155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGAT150.00221802270.0478346
AGGTGGT150.00221802270.0478346
TCTCGGG301.0180702E-770.0478343
CCGGGTT150.00221802270.0478343
TGAGCGA150.00221802270.0478340
TGATCAG150.002218989470.0401249
CGACGTA207.9010206E-570.0401253
AGCCCTG150.002218989470.0401254
GTATCAG252.8304385E-670.03242557
GGATCAT150.00221995770.03242557
TAGCAGT150.00221995770.03242558
GCGGAAA150.00221995770.03242558
GAATCAT252.8304385E-670.03242557
GGTAGCA150.00221995770.03242556
GTAGCAG150.00221995770.03242557
ATCAGTA207.9096295E-570.0247359
GCAGTAA207.9096295E-570.0247360
AAGAGTG150.002221893370.0170432
CAAGAGT150.002222861970.0093431
CTTGGGT150.002222861970.0093431
Produced by FastQC (version 0.11.5)