FastQCFastQC Report
Thu 26 May 2016
SRR1781380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455597
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT23914452.490249057829615No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG363167.971079704212275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195324.287122171568294No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT175333.848357210429393No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC134492.9519509566568702No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC66221.4534775250934489No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT58361.2809566349207742No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT43300.9504013415364896RNA PCR Primer, Index 45 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT38220.8388992903816311No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC24100.5289762663055287No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT21750.4773955930350727No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTATCTCGTATGCCGTC17130.3759901843076227No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG16660.36567404965353156No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT16300.357772329492951No Hit
TCATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG13720.30114333500879065No Hit
AATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT13260.2910466925813822No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC12940.2840229413275329No Hit
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC12590.27634071339363514No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC12150.2666830554195923RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT11150.2447338327513131No Hit
ATAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT9910.21751679664264692No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG9290.20390827858831378No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT9150.2008353874147547No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG8930.19600655842773326No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC8890.19512858952100212No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT8590.18854382272051837RNA PCR Primer, Index 45 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT7880.17295987462604012No Hit
TCTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT6250.13718264167674502No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACATTTATCTCGTATGCCGT5860.12862244483611612No Hit
TCTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG5480.12028174022217003No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT4580.10052743982071875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA150.002220749470.026141
CTGATAC150.002220749470.026141
CTAAACA150.002220749470.026141
TCTCATA150.002220749470.026141
CTTCTTA150.002220749470.026141
TCTTAAT1550.070.026141
TCCTTAT353.6961865E-970.026131
ATTATAA252.8319537E-670.026131
AACTTAT252.8319537E-670.026131
GTAGATC150.002222684470.010764
AGGGGTG150.002222684470.0107670
CTTCAAA207.917458E-570.0107657
TTCAAAA207.917458E-570.0107658
GAAAACA455.456968E-1270.0107660
CGTAGAT150.002222684470.010763
AGAAAAC150.002222684470.0107659
TGAAAAC301.0218173E-770.0107659
GTTGCAG150.002222684470.010763
CGTTTAT150.00222462169.9953932
ATCCCAC150.00222462169.9953917
Produced by FastQC (version 0.11.5)