FastQCFastQC Report
Thu 26 May 2016
SRR1781379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275604
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22709082.39720758769829No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG32121.1654402693719974No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5020.1821453970189112No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3910.14187021959042684No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.13933034353637827No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3550.12880799988389138No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3410.12372824777579425No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3260.11828565623140448No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT2770.10050652385306454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCA150.002220407670.0214269
GCGGCTA150.002220407670.0214266
GCGGTCT150.002220407670.0214238
GTCTGTT150.002220407670.0214241
TAATGCC150.002220407670.0214258
TCAACAA150.002220407670.0214233
TGGAGAT150.002220407670.0214237
GTATAGT150.002220407670.0214252
TCTGTTA150.002220407670.0214242
TAGTAAT150.002220407670.0214255
TCTCGGG207.905944E-570.0214243
ATAGTAA150.002220407670.0214254
GCCGGAT150.002220407670.0214254
GTTAGTA150.002220407670.0214245
GGTAGCA150.002220407670.0214256
CTAGCAC150.002220407670.0214270
AGGTTGC150.002220407670.0214233
GGCTAGC150.002220407670.0214268
CGGCTAG150.002220407670.0214267
CTGTTAG150.002220407670.0214243