FastQCFastQC Report
Thu 26 May 2016
SRR1781379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275604
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT9551134.655157399747466No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT4265715.477641833935646No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG117834.275337077836316No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC73272.6585245497162595No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT57382.081972685447236No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT55992.0315380038025572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46591.6904689336874648No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG36581.3272666579585202No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT34191.2405480326845764TruSeq Adapter, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC21360.7750250359211042No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT19750.7166078866779872No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC18440.6690759205236498No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT14650.5315597741687349No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT9520.3454231433506045No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT8040.291722906779292No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT8010.29063438847041406No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6830.24781933498788117No Hit
TCATTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC6150.22314625331998086No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC5600.203190084323885No Hit
TCAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG5540.20101304770612907No Hit
CATAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT5410.1962961350343246No Hit
ATTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT5080.1843224336366671No Hit
CTCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC5070.18395959420037444No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4900.17779132378339937No Hit
TCTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4390.1592865125324741No Hit
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC4350.15783515478730353No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT4300.15602095760584028No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4080.1480384900074019No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT4000.14513577451706072No Hit
CTAATACACAACTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT3620.13134787593793995No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC3500.12699380270242813No Hit
ATAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT3390.12300256890320894No Hit
CTAATACACATCTCCGAGCCAACGAGACTACACCTCATCTCGTATGCCGT3290.11937417454028244No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCCTCATCTCGTATGCCGT3090.1121173858144294No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT3080.11175454637813674No Hit
CTAATACACATCACCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT3020.10957750976038083No Hit
CTATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3000.10885183088779553No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGAATGCCGT2940.10667479427003963No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG2910.10558627596116167No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAACCTCATCTCGTATGCCGT2820.1023207210345278No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTAAGCCGT2810.10195788159823516No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGACGT2810.10195788159823516No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCACATCTCGTATGCCGT2760.10014368441677189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAGG207.91215E-570.01034566
AGGGGCG252.832774E-670.01034570
TTGTAAA252.832774E-670.01034558
CAACTTT150.002221802770.01034560
TGATCCC150.002221802770.01034570
AACTTTC150.002221802770.01034561
AAAGGGT150.002221802770.01034568
TTGATAA353.6961865E-970.01034558
CAAAGGG207.91215E-570.01034567
CTTTGAT150.002221802770.01034567
ACAACTT150.002221802770.01034559
TGTAAAA252.832774E-670.01034559
TCTTTAT2450.069.997651
ACTAATG207.919266E-569.9976450
AACGTAT207.919266E-569.9976439
AATCTGC455.456968E-1269.9976451
GTCATAT207.919266E-569.9976449
TATACGA207.919266E-569.9976411
AAATCAG207.919266E-569.9976410
TTCCGTC207.919266E-569.9976445
Produced by FastQC (version 0.11.5)