FastQCFastQC Report
Thu 26 May 2016
SRR1781378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893463
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17308319.372150833330533No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG33210.3716997793976919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA5600.053.7657856
CCGTATC5900.050.43853855
TCGCCGT6200.049.68922852
CGCCGTA6050.049.18523853
ATCTCGG6300.047.25726742
GTGGTCG6850.047.02110348
CGGTGGT7400.046.8538246
GATCTCG6400.046.5188741
GCCGTAT6400.046.49802454
TCGGTGG7400.046.3805545
TCTCGGT7000.045.53376443
GGTCGCC7000.045.5108150
CTCGGTG7200.045.2393544
GTAGATC6600.045.10920738
GTCGCCG7200.044.24662851
TGGTCGC7350.042.3910249
TAGATCT7500.040.63012739
TGTAGAT7650.040.29131737
GTCGGTA350.001254152240.00950659
TTGTGCG350.001257956439.9848672
Produced by FastQC (version 0.11.5)