FastQCFastQC Report
Thu 26 May 2016
SRR1781378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893463
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT8976310.046638752807894No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG173531.9422180884938718No Hit
ATTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT120441.3480132921005124RNA PCR Primer, Index 47 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60650.6788193803212892No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC45490.5091425162541706No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC39860.44612927451948203No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT34080.3814371719925727RNA PCR Primer, Index 47 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT33740.37763175419687217Illumina PCR Primer Index 9 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT20880.23369742227713963RNA PCR Primer, Index 47 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT14760.16519990195452974RNA PCR Primer, Index 47 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC14200.1589321549969053RNA PCR Primer, Index 47 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG10390.11628909087449621No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT9540.10677554638524483No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT9080.1016270399557676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG142400.064.22694470
CTCGAAG1150.054.78203630
AAGGGGG173400.052.5225869
GGGTCGT200.00694874152.50533363
CGTCAAC407.482995E-752.49945448
TCGTCGC200.006953356352.4965239
ATTATAC21050.052.380621
TCTCGAA3450.050.7212738
GATCTCG177500.050.2417336
ATCTCGT175450.050.0308337
ACTCGTA1400.049.99668538
GCCGTCT179000.049.95478446
CCGTCTT176700.049.911847
TGCCGTC180500.049.90804745
AGATCTC178550.049.8678735
CGCAGAT180800.049.86673732
TATGCCG180700.049.8528143
ATGCCGT181150.049.8255744
CCGTCTA2600.049.80438647
TCGCAGA183200.049.71018231
Produced by FastQC (version 0.11.5)