FastQCFastQC Report
Thu 26 May 2016
SRR1781377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237331
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38186330.86183082780598No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG120300.9722539886255174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG1150.054.756922
CGACGTA200.00694393752.51571353
TACGTCG200.00694393752.51571350
TTATGCG407.5090975E-752.475382
CGTCTGG2150.052.058079
TGCGCGT1300.051.1153955
CGTATCA19600.049.653756
TCGGTGG22050.048.915445
CGCGTCT1650.048.7531747
CCGTATC19650.048.63657855
CGGTGGT21050.048.57739646
TCTCGGT20800.048.32336843
CGCCGTA20200.048.18273553
CTCGGTG21150.048.0185144
TCGCCGT20300.047.94538552
GTGGTCG21700.047.9214448
ATCTCGG20500.047.8346742
GATCTCG20550.047.71828541
GGTCGCC21500.047.38627250
TCTGGCG2900.047.04309511
Produced by FastQC (version 0.11.5)