FastQCFastQC Report
Thu 26 May 2016
SRR1781377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237331
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT22802518.428779364616258No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG360832.9161962320510844No Hit
ATTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT198651.6054717775599254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192511.5558488391545997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC122460.9897109180970978No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC67340.5442359401001026No Hit
CTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT59470.4806312942939278No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT50450.407732449926495Illumina PCR Primer Index 6 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT37460.3027484157432409No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC27040.21853489486645042No Hit
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT23020.18604560946100923No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT21120.1706899770554524No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG18810.1520207608150123No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT15120.12219850630106252No Hit
TCATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG14730.11904656070202718No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC13980.11298512685772844No Hit
AATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT13680.11056055332000896No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC13620.11007563861246505No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC12380.10005406798989115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG150.002223915470.008166
AGGGGGG328000.065.9127770
ATTATAC35950.058.3309861
TACGAGA900.058.32834613
ACTCGTA4950.057.972538
TAACTCG4800.057.5969236
AACTCGT4950.057.2655237
AAGGGGG389650.055.33138369
CAACGCA4600.054.78014428
TCTCGAA6150.054.62727438
TATCACG3250.053.8393836
GCCGTCT387050.053.21898346
CCGTCTT383750.053.18418547
TATCTCG399650.053.08227536
TATGCCG395000.053.0781343
ATGCCGT395000.053.0426944
TGCCGTC393200.053.02740545
CTGCTTG382750.052.9891254
GTATGCC396350.052.9326642
CTCGTAT400200.052.93062239
Produced by FastQC (version 0.11.5)