FastQCFastQC Report
Thu 26 May 2016
SRR1781376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495270
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37462375.64015587457347No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG108242.185474589617784No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT11070.2235144466654552No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9180.18535344357623115No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6840.13810648737052517No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6270.1265976134229814No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6160.12437660266117471No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5160.10418559573565934No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5030.10156076483534235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATTG150.002220959670.0253743
TAATTGC150.002220959670.0253744
TGCGTCT150.002220959670.0253741
TATCAGT207.913914E-570.01829558
CGTATAA207.913914E-570.01829556
GCAAATT207.913914E-570.01829569
CTACGCA150.0022218570.01829561
GTATCAG207.913914E-570.01829557
CGTGTCA150.0022218570.01829556
CTCTACG150.0022218570.01829559
TGTTCGA150.002222740670.0112232
AGTTCGT150.002225414269.99000529
GAGTTCG150.002225414269.99000528
CTTGTAC150.00222630669.982931
TGATTCT150.00222630669.9829324
GGAGTTC150.00222630669.9829327
CCAATGT207.93771E-569.9758716
CAATTGC150.002227197969.9758716
GAATAGG150.002227197969.9758715
CCCAATG207.93771E-569.9758715