FastQCFastQC Report
Thu 26 May 2016
SRR1781376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495270
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT23515747.48056615583419No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG363327.335796636178246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174573.5247440789872195No Hit
ATTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT166603.363821753790862No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC120522.433420154663113No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC68131.3756133018353627No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT60191.2152967068467704No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT54101.0923334746703819RNA PCR Primer, Index 20 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT38330.7739212954550043No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC25820.521331798816807No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT23450.4734791124033355No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG15550.31397015769176406No Hit
TCATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG13430.2711652230096715No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC13180.26611747127829266No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC13000.2624830900316999No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC12960.26167544975467927No Hit
AATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT12880.260060169200638No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT11540.23300421992044743No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG11200.2261392775657722No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT10940.22088961576513821No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT10850.21907242514184183No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGCCATCTCGTATGCCGT10120.2043329900862156No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT9670.19524703696973367No Hit
ATAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT9380.18939164496133423No Hit
CAAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT8170.16496052658146063No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG7870.158903224503806No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC7320.14779817069477255No Hit
TCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT7110.14355805924041432No Hit
TCTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG5900.11912694086054071No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCATCTCGTATGCCGTC5350.10802188705150725No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT5340.1078199769822521No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC5310.10721424677448664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGT150.002220949670.0254564
ATACGGG150.002220949670.0254562
CTTAGAA150.002220949670.0254563
AATACGG150.002220949670.0254561
TATCCTC150.002220949670.0254561
GTTTGGC150.002221839770.0183869
GTTAATA150.002221839770.0183858
TGCTCGC150.002221839770.0183868
GCTCGCC207.9138685E-570.0183869
GGTTAAT150.002222729870.01131457
CTATCAG207.917828E-570.011313
CGGTTAA150.002223620270.0042456
TGCTTTA401.3460522E-1069.9971855
CACGACA207.925753E-569.9971821
GACGCAA650.069.9971826
CGGAAAA150.00222451169.9971722
CAAACGA353.7089194E-969.9971719
GCAAACG353.7089194E-969.9971718
TTCGTAG150.00222451169.9971718
AGTTGGG150.00222451169.9971717
Produced by FastQC (version 0.11.5)