FastQCFastQC Report
Thu 26 May 2016
SRR1781375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580408
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44421176.53426555112955No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG141282.4341497705062647No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT14830.2555099171617207No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11890.20485589447423194No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8790.15144519027994102No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8290.1428305605711844No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8010.13800636793428073No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7140.12301691224104423No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG6660.11474686772063789No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5870.10113575278080247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGT207.898409E-570.04806543
GTCAATG150.00221905970.0420237
CCGTGTA150.002219818570.0359834
ACCTCCT150.002219818570.0359836
GAGCGTA150.002219818570.0359858
CCTGGTG150.002219818570.0359835
CGGTAGC150.002219818570.0359855
GACTCTT150.002219818570.0359845
TGGCGTA150.002220578370.0299468
AGCGTAG150.00222133870.02389559
TAAACAG150.00222133870.02389533
ATATGTT207.911922E-570.02389559
AGCCTGG150.00222133870.02389533
TCGGTAA150.00222133870.02389560
CAGTAAG150.002222098270.0178561
CGGTAAG150.002222098270.0178561
ATGTTGT207.915303E-570.0178561
ATGTAGC150.002225140369.99370629
CGTATAC207.9356134E-569.981641
CGTGTGC150.002226662869.9816361