FastQCFastQC Report
Thu 26 May 2016
SRR1781375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580408
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT26710546.02021336714863No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG422817.2847031743187545No Hit
ATTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT235354.054906203911731No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228143.930683243511461No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC142152.449139226199501No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC89191.5366776474480022No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT70351.2120784000220535No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT63781.0988821656489918No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT47090.8113258259706965No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC34150.5883792091080757No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT28840.4968918416010806No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT25640.44175821146503835No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG25610.441241333682513No Hit
TCATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG18260.314606276963791No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC18060.31116042508028835No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT17480.3011674546181307No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC17270.29754931014045294No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC16900.291174484155973No Hit
AATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT16770.28893468043169634No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG14230.24517236151121283No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAAATATCTCGTATGCCGT13610.23449022067235462No Hit
ATAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT13420.2312166613830271No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT12390.21347052418298854No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC11820.203649846315006No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11390.19624126476547535No Hit
CAAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT9670.16660693856735262No Hit
TCTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT9040.15575250513431932No Hit
TCTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG7280.12542900855949607No Hit
CTAAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT5970.1028586787225538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCT207.905956E-570.0345769
TCTTTAC150.002222274470.0164641
ATAGGCG207.919469E-570.010433
ACGCATC455.456968E-1270.010436
GCACATC150.002223034270.010436
TACGCAT455.456968E-1270.010435
CTAAACC150.002223034270.010434
ATACGCA455.456968E-1270.010434
TTATACG500.070.010422
TATACGC500.070.010423
GCTACAA150.002223793970.00439556
ACCGATC150.00222455469.9983613
AATACGA252.8396153E-669.9983611
CTTACCT207.9262325E-569.9983611
ACGCACG150.002225314569.9923331
ACGCAAT252.8410686E-669.9923330
AGCCCCA252.8410686E-669.9923317
TACGCAC150.002225314569.9923334
GCTTACC207.929616E-569.9923310
CGCAATA150.002225314569.9923331
Produced by FastQC (version 0.11.5)