FastQCFastQC Report
Thu 26 May 2016
SRR1781374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612249
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47982778.37121824617108No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG46180.7542682797358591No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17130.27978812541955966No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11500.18783207485843179No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10410.1700288608066326No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9960.16267891005130264No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8620.14079239002432017No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8130.13278911031296092No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6860.11204591595902974No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6180.10093932370653118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT150.002216895370.05971543
GGATGTC207.892254E-570.05971540
GATCACG150.002216895370.05971541
GTGGATC150.002216895370.05971538
TAGATCA252.825058E-670.05971539
TAGATAA150.002216895370.05971540
GGTTAAC150.002216895370.05971538
TGTGGAT455.456968E-1270.0539837
CGTGGTT207.905053E-570.036835
GTGCAGT207.9114594E-570.02534556
GGGTAGC207.9114594E-570.02534555
GAATAGT207.9114594E-570.02534551
GTAGCAT301.020926E-770.02534557
AGGCACT150.00222121470.02534556
ACGGATA150.00222121470.02534555
GTGTCAT301.020926E-770.02534557
ATAGTGC207.9114594E-570.02534553
TAGTGCA207.9114594E-570.02534554
GGTAGCA150.00222121470.02534556
TATCATG150.002221934370.0196258
Produced by FastQC (version 0.11.5)