FastQCFastQC Report
Thu 26 May 2016
SRR1781374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612249
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT35583758.119653931651996No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG394166.437903532712998No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC180322.9452069337802103No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT89691.4649268516567606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80041.3073112410146852No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC71071.1608022226251085No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT48900.7986946487458534RNA PCR Primer, Index 17 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT46100.7529616218238004No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC34840.5690495207015447No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT30910.5048599507716631No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT24310.3970606730268241No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG24180.39493735391972873No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAACAGATCTCGTATGCCGT19980.3263378135366493No Hit
ATTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT18370.30004132305646886No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC15270.24940832896419596No Hit
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC15110.24679501314007862No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTACAGATCTCGTATGCCGT14650.23928173014574136No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC14330.23405509849750675No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12740.20808527249534095No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG11860.19371203546269575No Hit
CTTATACACATCTCCGAGCCAACGAGACAAATACAGATCTCGTATGCCGT11220.1832587721662265No Hit
TCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG10540.17215217991372792No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCG10220.16692554826549327No Hit
CTAAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT9410.1536956369058994No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT9220.15059232436476008RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAATAAAGATCTCGTATGCCGT9160.1496123309307161No Hit
CTTATACACATCTCCGAGACCACGAGACAAATACAGATCTCGTATGCCGT8980.14667235062858414No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGACGT8980.14667235062858414No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCAGT8690.14193571569737148No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT7500.12249917925549898No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAATCTCGTATGCCGTC7180.11727254760726437No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGA6920.11302590939307373No Hit
CTTATACACATCTCAGAGCCCACGAGACAAATACAGATCTCGTATGCCGT6840.11171925148101508No Hit
CAAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT6430.10502262968171447No Hit
CTTATACACATCTCCGAGCACACGAGACAAATACAGATCTCGTATGCCGT6380.1042059684866778No Hit
TCTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT6280.10257264609660449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC150.002220691970.02951
CTTTACA1650.070.0294951
CTCTATA500.070.0294951
TCTATAT252.8323102E-670.0294951
CACACTG150.002221411870.023785
TTCCTAC150.002221411870.023783
TATCCAC150.002221411870.023783
CTGGACC150.002221411870.023784
ATGCTGG150.002221411870.023786
TGGACCT150.002221411870.023785
TGGTCTT207.912341E-570.023775
AGCTTAT207.912341E-570.023772
ACTGGTC207.912341E-570.023773
TTTACAC1700.070.023772
ACGGGGT150.002223572270.0066270
GGGTTAT207.921953E-570.00661562
TTTTCCT207.921953E-570.00661570
GGTTATC207.921953E-570.00661563
AGGTGGG401.3460522E-1070.00661570
TCATGAA150.002224292670.000956
Produced by FastQC (version 0.11.5)