FastQCFastQC Report
Thu 26 May 2016
SRR1781373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587807
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47233680.35562693196917No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG73051.242754849806144No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12110.2060200031643039No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9030.15362185207049253No Hit
TCTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGT8340.14188330523454126No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7230.12299955597670664No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6900.11738546835951257No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6820.11602447742201097No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6220.10581704539074901No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6050.10292493964855812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCT252.8302711E-670.03738439
GGATGTC252.8302711E-670.03738440
CGTATGA150.002221155270.0254656
GGATCAG252.833136E-670.0254657
CTACACA150.002221155270.0254634
AAGTGTA150.002221155270.0254634
GGGATCA150.002221155270.0254656
AGATGGA150.002221155270.0254647
GTGTGTA353.6980055E-970.0254534
ATCAGGA252.8345694E-670.019559
GATCATT150.002221905470.019558
TGATTAA150.002221905470.019560
GGATTCA150.002221905470.019549
AATCATT207.914471E-570.0194958
GCATTAA207.914471E-570.0194960
AGCATTA207.914471E-570.0194959
CTTGTGG150.00222265670.013541
ATTAAAG150.00222265670.0135462
GTTGTAG150.00222265670.013541
CAAGGAA150.00222265670.0135466
Produced by FastQC (version 0.11.5)