FastQCFastQC Report
Thu 26 May 2016
SRR1781373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587807
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT32744155.70552919580747No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG365406.216326107038535No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC169702.8870020261752583No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT138362.3538338264090086No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110841.88565294390846No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC63641.0826682907825187No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT63341.0775645747668878No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT37140.6318400427351154RNA PCR Primer, Index 43 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT31950.5435457556646994No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC28780.48961648976619876No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT28340.48213103960994No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG20750.3530070244144762No Hit
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT20560.34977467093790987No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT19190.32646770113319507No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG15500.26369199414093397No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTAGTATGCCGT15160.25790778265655223No Hit
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC13790.2346008128518374No Hit
ATTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT13460.22898672523464333No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGAATCTCGTATGCCGT13440.22864647750026795No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC13420.22830622976589254No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGACGT13080.22252201828151078No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC12910.2196299125393199No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG12590.21418594878931352No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAACAGCATCTCGTATGCCGT11160.18985823578147248No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG10660.18135204242208752No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCC9940.16910312398457317No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGA9190.1563438339454957No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCCGCATCTCGTATGCCGT8640.14698702125017224No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT8040.13677958921891029RNA PCR Primer, Index 43 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACAGACCAGCATCTCGTATGCCGT7870.13388748347671942No Hit
TCATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG7330.12470079464858363No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGAATGCCGT7290.12402029917983283No Hit
CTTATACACATCTCCGAGACCACGAGACAGACCAGCATCTCGTATGCCGT6740.11466348648450936No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCAGT6160.10479630218762281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACA207.918149E-570.012931
AACCGGG150.002222732970.0129369
TTTGTCC150.002222732970.0129367
AACTTCC150.002222732970.012931
AGGACCT150.002222732970.0129365
TTGAGTT150.002222732970.0129365
AACGCAC150.002222732970.0129365
AGGGGCG252.836152E-670.0129370
TTTTGTC150.002222732970.0129366
TAGGACC150.002222732970.0129364
CTGATAC500.070.012931
CTTACAC207.918149E-570.012931
GATACAC500.070.006983
CCTAGGA150.00222348370.0069862
CCCTAGG150.00222348370.0069861
CACATAC150.00222348370.006983
TGAACAA207.9214864E-570.0069859
TGAAAAC252.8375853E-670.0069859
TTATATA353.7071004E-970.006982
TCTACAC207.9214864E-570.006983
Produced by FastQC (version 0.11.5)