FastQCFastQC Report
Thu 26 May 2016
SRR1781372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710476
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40148656.50943874247687No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG32220.45349878109886893No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10400.14638073629510356No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8100.11400807346060951No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA150.00222207770.0196559
GTATACG207.9431746E-569.970362
GACGGCC150.002229536969.9605123
TCATGCG150.002230781869.950674
CGCGTGT301.02872946E-769.950677
GTACGCG150.002230781869.950674
GGTCTCG900.058.37438241
GTCCCAT308.376854E-658.3332751
TTGTGCG1050.056.6426772
CGTCTGG2850.055.2242139
CGCGTCT1850.054.8261957
GTGCGCG2600.053.80824
TGTGCGC1650.052.992933
GCGATGT200.00693163452.5369540
CCGGGAG200.006933566652.5332536
CGTGGCA200.006939365552.52214456
TATCGTT200.00694323452.5147458
ATTCTCG200.00694516852.51104461
GTATGCG200.00696259852.477772
ACGACAC200.00696453752.4740827
Produced by FastQC (version 0.11.5)