FastQCFastQC Report
Thu 26 May 2016
SRR1781372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710476
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT29157741.03966917953598No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG325924.587347074355784No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC164892.320838423817272No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT67350.9479560182187716No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC58770.8271919107753113No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57630.8111463300660402No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT44280.6232441349179986RNA PCR Primer, Index 24 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT34910.4913607215444294No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC28090.3953687387047557No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT24420.3437132288775413No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT22700.31950410710565874No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG18580.26151481541952154No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT17080.24040220922311237No Hit
ATTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT15280.2150670817874214No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC14350.20197726594564772No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT13240.18635393736030492No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC11670.16425607620806335No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC11540.16242631700437452No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG11470.1614410620485421No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG11190.15750004222521238No Hit
CTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCGT10280.14469172779939082No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT8380.11794909328393921No Hit
TCATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG8130.1144303255845377No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACGT8090.11386732275263346No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCAGT7750.10908179868144736No Hit
CTTATACACATCTCCGAGACCACGAGACGACTTAGTATCTCGTATGCCGT7370.10373327177835703No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAT150.002222558370.0158465
TCCGTCC150.002223178770.0109159
GTTGACT150.002224420470.001063
CACGGTT150.002225041469.9961332
TCGGGAG150.002225041469.9961355
CCGCTTT150.002225041469.9961325
TGGCATG150.002225041469.9961337
CACTCGA150.002225041469.9961337
ACTCGAT150.002225041469.9961338
ATCGCAT150.002225041469.9961327
CGAAAGA455.456968E-1269.99612441
AGGGGGG364100.067.0736770
CGAGCAA850.065.8787115
TCGAAAG1400.062.49654440
CCGTCTA4050.062.2187847
AAGCCGT5350.060.18358644
ATCGAAT352.9754847E-759.9966839
CGAAAGC1050.059.99667741
CTTAAAC10800.059.967271
TCGTAAG6250.059.91668740
Produced by FastQC (version 0.11.5)