FastQCFastQC Report
Thu 26 May 2016
SRR1781371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674992
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46517468.91548344276673No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG47850.7088972906345556No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14020.2077061654064048No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8810.1305200654229976No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8160.12089032166307156No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7500.11111242799914667No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7390.10948277905515917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGA150.00221510970.07474538
CGCGGTA252.8304967E-670.0383553
TAGCGCT150.002219677270.0383551
TGCGTCA150.002222944570.0123863
GTTCACC150.002224906669.9968166
CGAAGGT353.7271093E-969.960525
TATGCGG150.00223211269.9397663
ATCGGAC150.00223211269.93976610
TTATGCG650.064.574142
TTGTGCG1150.063.872252
ATGCGCG501.4551915E-1162.945794
GTTATGC453.8380676E-1062.2378431
CTTATGC700.060.0150641
CGACGTA308.3492E-658.36529553
TCGACGT308.3492E-658.36529552
GACAGCG308.419202E-658.2831427
ACAGCGG308.419202E-658.2831428
GTACGCG308.419202E-658.2831424
GGTGTAG1950.057.47589535
CGGTGTA553.6379788E-1157.30410834
Produced by FastQC (version 0.11.5)