FastQCFastQC Report
Thu 26 May 2016
SRR1781371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674992
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT33214749.20754616351009No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG387735.744216227747884No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC175382.5982530163320456No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79871.1832732832389126No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC75991.1257911204873539No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT72151.0689015573517908No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT50060.7416384194183042RNA PCR Primer, Index 45 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC35750.5296359067959323No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT35620.5277099580439472No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC35390.5243025102519733No Hit
CATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT24560.3638561642212056No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG24420.3617820655652215No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT20870.30918884964562543No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT18710.27718847038187117No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC17810.2638549790219736RNA PCR Primer, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC16080.2382250456301704No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG15550.23037310071823075No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT14910.22089150686230358No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC14790.2191137080143172No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT14730.218224808590324No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT14660.21718775926233197No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC14470.21437291108635362No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG13820.20474316732642756No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT9950.14740915447886788No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAAATCTCGTATGCCGT9780.14489060611088722No Hit
CTTATACACATCTCCGAGCCAACGAGACTCTTATTAATCTCGTATGCCGT9550.1414831583189134No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGACGT9090.13466826273496577No Hit
TCATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG8530.12637186811102946No Hit
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT7530.11155687771114324No Hit
CTTATACACATCTCCGAGACCACGAGACTCTTATTAATCTCGTATGCCGT7490.11096427809514778No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCAGT7200.10666793087918079No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGA7020.10400123260720127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG150.002222890370.0128267
GGTATGC150.002223543670.0076357
ATGCGTG150.002223543670.0076358
GTCTACG150.002224197170.0024433
CTACGTG150.002224197170.0024435
TATGGAC150.002225504569.992078
CACGATA301.0244548E-769.9920721
TAGTTCG150.002225504569.9920747
GTTCGTG150.002225504569.9920749
GTTGTAC150.002225504569.992078
AGTTCGT150.002225504569.9920748
CGATACT207.930697E-569.99206523
AGGGGGG422050.066.4877170
CTTAAAC9600.065.291721
GACAACG1200.064.159418
CTCTATA501.4551915E-1163.030221
TCCGATC501.4551915E-1162.99286713
AGACGTC501.4551915E-1162.99286745
TCTTTAT9150.062.762331
CTAATAC18850.061.488371
Produced by FastQC (version 0.11.5)