FastQCFastQC Report
Thu 26 May 2016
SRR1781370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584997
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47198680.681781274092No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG41070.70205488233273No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10720.18324880298531446No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8420.1439323620463011No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7480.1278639035755739No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7170.12256473110118513No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5870.10034239491826452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCT207.906623E-570.0334639
GTAGAGC207.906623E-570.0334638
AGGGGTC207.906623E-570.0334640
TGTAGAG301.0200165E-770.0334637
CCACACG150.002220143870.0334636
TGTAGCC150.002220897570.02746634
AAGTGTA252.8326358E-670.02746634
CTGTGTA353.6980055E-970.02746634
TGTCATT150.002221651170.0214858
CCGATAA150.002221651170.0214853
GCAGTGA252.8340764E-670.0214860
ATTAAAT150.002221651170.0214862
GGTCAGT150.002221651170.0214858
TGTGATG150.002221651170.0214858
GTGTGAT207.9133286E-570.0214857
CAGTGAA207.9133286E-570.0214861
CAGTAAA353.6998244E-970.0214861
CGGTAGA150.002221651170.0214861
GCGTATG150.002221651170.0214855
TCGGTAA150.002221651170.0214860