FastQCFastQC Report
Thu 26 May 2016
SRR1781370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584997
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT34523159.01414879050662No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG391686.695418950866415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC197573.377282276661248No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT72691.2425704747203832No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71401.2205190795850236No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC70201.200006153877712No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT46790.7998331615375806Illumina PCR Primer Index 10 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT36250.6196612974083627No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC34080.5825670900876415No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG27090.4630792978425531No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT25360.4335064966145125No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT25150.42991673461573304No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT16230.2774373201913856No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTAATCTCGTATGCCGT16020.27384755819260614No Hit
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC15900.271796265621875No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG15430.26376203638651136No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGACTTATCTCGTATGCCGT14400.24615510848773584No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC14110.24119781810846894No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC13850.23675335087188482No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT13220.22598406487554637No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG12550.2145310146889642No Hit
CTTATACACATCTCCGAGCCAACGAGACTGTGCCTTATCTCGTATGCCGT9910.16940257813287932No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGCCTTATCTCGTATGCCGT9840.16820599079995283No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT9450.16153928994507666No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG8990.15367600175727397No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGACGT8660.14803494718776336No Hit
CTTATACACATCTCCGAGACCACGAGACTGTGCCTTATCTCGTATGCCGT8460.1446161262365448No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCAGT7080.12102626167313679No Hit
TCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT6960.11897496910240565No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCATATCTCGTATGCCGT6630.11333391453289504No Hit
CTAAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT6510.11128262196216392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT150.002221502870.022661
CTTTTAC150.002221502870.022661
AGTGGTG150.002221502870.0226670
CTTAGAC150.002221502870.022661
AAGTGGT150.002221502870.0226669
AGGGAGG150.002221502870.0226670
CTTACAC150.002221502870.022661
TAAAAAG150.002222256370.0166765
CTTTAAA500.070.0106957
TAGACAC150.002223010270.010683
GACACAT150.002223010270.010685
TTAGACA150.002223010270.010682
TTATACG150.002223010270.010682
CCACTCT150.002223010270.010684
GTCCACT150.002223010270.010682
TTTTATA150.002223010270.010682
TTTTACA150.002223010270.010682
GCTTAAA301.0229269E-770.004756
GCTACAA150.00222376470.004756
GTTTCAC150.002224518269.9987236
Produced by FastQC (version 0.11.5)