FastQCFastQC Report
Thu 26 May 2016
SRR1781369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583350
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46555979.80783406188394No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG44810.7681494814433873No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13770.23605039856004115No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9330.15993828747750063No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8350.14313876746378673No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8000.13713893888746034No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7400.12685351847090084No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6920.1186251821376532No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6240.10696837233221909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCG207.8973375E-570.0500541
TAGAGCT252.8272098E-670.0500439
CAAGTGT150.002218056770.0500441
ACGTTTT150.002218056770.0500437
TTATGCC150.002218056770.0500437
CTAAGGC150.002218056770.0500443
CCTAAGG150.002218056770.0500442
TATGCCT150.002218056770.0500438
GCCTAAG150.002218056770.0500441
TAGATCG150.002218056770.0500439
ATTATGC150.00221881270.0440336
CCTCCTA150.002219567370.0380235
TAAGGCC150.002219567370.0380244
GCAGCAC150.002219567370.0380246
AAATCTC150.002221078870.02634
CAGCACG150.002221078870.02647
CCGTGTA150.002221078870.02634
TCACGAC150.002221834870.0199950
GTCATTT150.002221834870.0199955
CGTTGGT150.002221834870.0199952