FastQCFastQC Report
Thu 26 May 2016
SRR1781369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583350
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT33453157.346532956201244No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG390486.69375160709694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC174562.992371646524385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75901.3011056826947802No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT72411.2412788206051255No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC69361.1889946001542813No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT48110.8247192937344647RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC39090.6700951401388532No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC36790.6306676952087084No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT36340.6229536298962887No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG25350.43455901259963997No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT24870.42633067626639237No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT23970.4109025456415531No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT21240.36410388274620725No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC16400.28113482471929374No Hit
ATTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT14940.2561069683723322No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC14150.2425644981571955No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT14090.24153595611553957No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG13910.2384503299905717No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC13840.2372503642753064No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG12980.22250792834490443No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAAATCTCGTATGCCGT12010.20587983200479987No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCTTAATCTCGTATGCCGT10700.18342333076197823No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACTTAATCTCGTATGCCGT10370.1777663495328705No Hit
CTTATACACATCTCCGAGCCAACGAGACCAATCTTAATCTCGTATGCCGT9820.1683380474843576No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT9770.16748092911631096No Hit
TCATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG9120.15633839033170482No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGACGT8230.14108168338047483No Hit
CTTATACACATCTCCGAGACCACGAGACCAATCTTAATCTCGTATGCCGT8210.14073883603325618No Hit
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT7640.13096768663752464No Hit
CTAAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT7080.12136796091540242No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCAGT6460.11073969315162424No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAATCTCGTATGCCGTC6260.10731121967943773No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGA5970.10233993314476729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGA252.833378E-670.024356
TTAGGCA150.002221286870.024354
GCTTGCT150.002221286870.024351
GCTTCTA150.002221286870.024351
TACGCAT252.833378E-670.024355
ACATGAC150.002221286870.024355
GATGAGA150.002221286870.024351
GTCTTTA150.002221286870.024351
CTTTACA1600.070.024351
GTCTTAG150.002221286870.024352
TAGACGC150.00222279870.01234461
TTTGTTC150.00222279870.01234465
ATAGACG150.00222279870.01234460
TGAAAAC401.3460522E-1070.01234459
CATAGTA150.00222279870.01234461
GTAGACA150.00222279870.01234461
ATTATCC150.00222279870.01234467
GCATAGT150.00222279870.01234460
GCGAAAT150.00222355470.0063568
AATGGTG150.00222355470.0063569
Produced by FastQC (version 0.11.5)