FastQCFastQC Report
Thu 26 May 2016
SRR1781368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536097
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43263280.7003210239938No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG40500.7554602991622784No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9910.18485460653575753No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8330.15538232819806863No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6400.11938138060836007No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6350.11844871357235724No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6330.11807564675795612No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5960.11117391069153529No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5470.10203377373870773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGAT207.8956335E-570.0520537
CTTATTT150.002217706370.0520537
TTCTCTG150.002217706370.0520542
GTTTCTT150.002218528470.045535
TCTTATT150.002218528470.045536
CATGCCC150.002219350570.03895655
CCGTGTC301.0193071E-770.03895655
TCAGGGG207.902946E-570.03895660
TACTGTT150.002219350570.03895634
GGGGTCA252.8295526E-670.03895656
GGTATCA150.002219350570.03895656
CAGGAAA301.0193071E-770.03895661
CGGGAGA207.902946E-570.03895661
CAGTGAA150.002219350570.03895661
CAGTAAG150.002219350570.03895661
GTCAGTA150.002220173170.0324259
TAGCAGG301.0199619E-770.0324258
GTGTCAG207.9066034E-570.0324257
GGGCAGT401.3460522E-1070.0324258
GCTACGT150.002220173170.0324267