FastQCFastQC Report
Thu 26 May 2016
SRR1781368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536097
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT31498258.75466566684761No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG360246.719679460993067No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC198093.6950402632359443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70491.3148739873567656No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT69401.2945418459719042No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC63611.1865390032027787No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT46020.8584267399369891RNA PCR Primer, Index 45 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT35680.6655511968916072No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC29170.5441179488040411No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT23910.4460013766165451No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG22620.4219385670876726No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT21420.3995545582236051No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTATCTCGTATGCCGTC21360.3984353577804017No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT16580.3092723891385327No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT15770.29416318315528717No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC12980.24212036254633024RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT12630.23559169329431057No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG12420.23167449174309873No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC11440.2133942178374436No Hit
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC11250.20985008310063288No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG10550.19679274459659352No Hit
CTTATACACATCTCCGAGCCAACGAGACGGTCATTTATCTCGTATGCCGT8670.16172446404288776No Hit
TCATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG8290.15463619456926636No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT8250.15389006094046412RNA PCR Primer, Index 45 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGACGT7770.14493645739483713No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACATTTATCTCGTATGCCGT7560.14101925584362532No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCAGT7480.1395269885860208No Hit
CTTATACACATCTCCGAGACCACGAGACGGTCATTTATCTCGTATGCCGT7140.13318485274120168No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAATTTATCTCGTATGCCGT6320.11788911335075555No Hit
CTTATACACATCTCAGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT6140.11453151202114543No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT5980.11154697750593644No Hit
TCTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT5810.10837590958352686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT150.00222043670.03033466
AGCGGGG500.070.02379670
AGGGGCG150.002221258170.02379670
AGGGAGG150.002221258170.02379670
GACGTAG150.002222080570.0172651
AGAAACA207.9150894E-570.01726559
AGTAGCG150.002222080570.0172651
CTGATAC207.9150894E-570.0172651
GTTATAC150.002222080570.0172651
TCTTAAT1750.070.017261
CGACAGG207.922409E-570.0041966
ACCCTGC150.002223725770.0041963
GTAGCCT150.002223725770.0041964
TACTCAT150.002223725770.0041966
CCGACAG207.922409E-570.0041965
GTACGCC150.002223725770.0041966
TAGACGC150.002224548569.99766524
GACACGG301.0234726E-769.99766524
GACGTTC252.8394861E-669.99766526
TACGGTC150.002224548569.99766526
Produced by FastQC (version 0.11.5)