FastQCFastQC Report
Thu 26 May 2016
SRR1781367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692962
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28285940.81883277870937No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG37110.5355272006257197No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8540.1232390809308447No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGG207.944188E-569.968328
GTACGCG150.002229164869.963284
CGCGTCT800.061.2222827
CGTCTGG1250.058.7733889
GATCGCG308.345347E-658.37011741
TTGTACG308.391911E-658.3153532
TATGCGC509.858923E-1055.970623
CGCCGTA6550.055.58748253
CCGTATC6650.054.75553555
TGTCGGT653.6379788E-1253.88010443
TTGTGCG850.053.512912
CGTATCA6750.053.42178756
TGCGCGT1050.053.305355
TCGCCGT6950.052.89193752
CCTCTCG200.006933571352.533145
TAGATCG200.00693555252.5293139
TCGGCTC200.00694348152.51413349
CACTCGT200.006947447552.5065559
TGCGCCG200.00695737252.48760230
CTCGAAC200.006961344752.48002616
Produced by FastQC (version 0.11.5)