FastQCFastQC Report
Thu 26 May 2016
SRR1781367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692962
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT13722819.80310608662524No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT503277.262591599539368No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC115501.6667580617696207No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG83961.2116104490578128No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT65760.9489697847789633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54510.7866232203208834No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT42190.6088356937321239No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT31130.4492309823626693TruSeq Adapter, Index 18 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG29920.43176970742984466No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT26770.38631266938158226No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT23100.3333516123539242No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC20250.2922238160245439No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG18680.26956745102906077No Hit
ACATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG15050.21718362623058696No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT12660.18269400053682597No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC10400.1500803795879139No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8970.12944432739457576No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT8790.12684678236324645No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT6980.1007270239926576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT150.002223193670.0106167
TGTTCGA150.0022238370.0055658
TCGAGCG150.0022238370.0055661
TCGAGTG150.002225103369.9954540
CGAAAGA252.8408977E-669.99544541
AGGGGGG274900.066.7125570
CTAATAC79150.064.403141
TAATACA79250.064.3079452
AATACAC94800.061.32873
ACTCGTA6650.060.52675638
GAACACG1400.059.996118
AAGGGGG306150.059.41840469
AACTCGT6600.059.39436337
CCGAGAA1650.059.3900814
AGCCGAC1500.058.32954845
CCGTCTT291700.058.033647
GCCGTCT296450.058.0245746
ATCTCGT295550.057.98045737
CGTCTTC293700.057.97206548
CCGTCTA4650.057.95322447
Produced by FastQC (version 0.11.5)