FastQCFastQC Report
Thu 26 May 2016
SRR1781366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642108
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40488663.0557476312396No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG28130.4380882966728339No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7860.12240931432095535No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7770.12100768095086809No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6790.10574545092102886No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGT150.002217911670.0520145
TTAGTCG207.918219E-570.01379467
TATCGTT150.002222717370.01379458
ATGCGCC207.921276E-570.0083468
CACGGTA150.002228907369.9647223
CGACCTT150.002228907369.9647225
CCCGACT150.002228907369.9647219
GTATCGA150.002230284569.953834
TATGCGC352.98749E-759.960423
CGGTGTA601.8189894E-1258.3584834
CGTCTGA308.404837E-658.2993939
ACGTCTG601.8189894E-1258.2993938
CGTATCG252.3879121E-456.0110456
CGTCTGG2000.055.9674159
TACGCCC252.3980449E-455.963064
TCTGGCG2650.055.4394211
TTATGCG452.7135684E-854.4127662
GTGCGCG1550.054.1578034
CTGCCGG3050.053.90724618
TTGTGCG1300.053.8148232
Produced by FastQC (version 0.11.5)