FastQCFastQC Report
Thu 26 May 2016
SRR1781366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642108
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT28596044.53456427890635No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG315714.91677412522504No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC139782.176892360786659No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT97271.5148541989821027RNA PCR Primer, Index 47 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT60000.93442224672485RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC58280.907635475652071No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51080.795504806045089No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT41740.650046409638254Illumina PCR Primer Index 9 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT31780.4949323166819289No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT26170.40756383661315543No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC23500.3659820466338996RNA PCR Primer, Index 47 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG21100.3286051567649056No Hit
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT20780.3236215714490397RNA PCR Primer, Index 47 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT15450.2406137285316489RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGCCCCTCGCAGATCTCGTATGCCGT13010.20261389049817166No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCG12540.19529424956549365No Hit
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC11530.17956480841229203No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC10670.16617142287590247RNA PCR Primer, Index 47 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG10390.16181078572451985No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC10220.1591632560254661No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCG10180.1585403078609829No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG9590.1493518224348552No Hit
CTTATACACATCTCCGAGCCAACGAGACCCTCGCAGATCTCGTATGCCGT7480.11649130675836464RNA PCR Primer, Index 47 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG7200.11213066960698201No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT6790.10574545092102886No Hit
CTAATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT6570.10231923601637108No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCC6520.10154055081076703No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAG150.002222091770.0187765
CCATAAC150.00222415270.002413
AGCGTAC150.002224838869.9969657
CATTCGG150.002224838869.9969610
ACGGGCT150.002224838869.9969612
TGCGATA150.002224838869.9969622
TGATTGT150.002224838869.9969614
ACGTATC150.002224838869.9969640
TGTCGTC150.002224838869.9969623
AGGGGGG366650.067.2869870
ACCTCGC3250.062.4588328
CTTAAAC8750.062.002141
CGACTAC1500.060.66403248
CCGTCTA3700.060.53791447
CTTTACA1800.060.2798581
CAGGTGG352.9747935E-759.99739517
TCCGATC352.9747935E-759.99739513
CGCATAT700.059.99739532
CATCGAA352.9747935E-759.99739529
CTAATAC16650.059.911981
Produced by FastQC (version 0.11.5)