FastQCFastQC Report
Thu 26 May 2016
SRR1781365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925709
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39578642.75490461905415No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG35040.37852067982486937No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10500.11342657357765777No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG150.00223259469.938042
CGGTAGC252.3872091E-456.01700255
TGTGCGC2000.055.9443863
CCGTATC6300.055.57242655
CGCCGTA6200.055.3393853
CGCGTCT1900.055.2261737
CGTATCA6650.054.75346456
TACACGT452.7193892E-854.4021265
TCGCCGT6700.054.34485652
TCTGGCG4150.053.94853211
CGTCTGG2800.053.7169349
GCCGTAT6650.053.17403854
TCTCGGT8200.052.96581643
TCGTGAC200.006943268752.5159454
AGGTTCG200.006943268752.5159457
GTCGTTA200.006943268752.5159456
TCCCGAA200.006943268752.5159458
CGATCTA200.006943268752.5159459
TATCCCG200.00696557152.47337317
GTACACG200.006977487452.4506954
Produced by FastQC (version 0.11.5)