FastQCFastQC Report
Thu 26 May 2016
SRR1781365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925709
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT28078830.332210230212738No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG314443.3967477900722582No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC150171.6222160527768446No Hit
CTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT63570.6867168840315909No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60480.6533370638073087No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC59860.6466394947008185No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT40130.4335055616829911Illumina PCR Primer Index 6 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT32380.34978594785186273No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC28090.3034430906472768No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT22380.24176063968266484No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT21280.22987785578405306No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG19400.2095690978482439No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT17280.18666773251637395No Hit
ATTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT13810.14918295058166228No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC13120.14172920431798763No Hit
CCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG12290.13276310373994418No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT12200.13179087596642142No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCAGT12120.13092667350106782No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC11970.12930629387852985No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC11720.12660566117429992No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATCTCGTATGCCGTCT11680.12617355994162313RNA PCR Primer, Index 41 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAAGCCATATCTCGTATGCCGT11210.12109637045767081No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG11160.12055624391682483No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGACATATCTCGTATGCCGT10920.11796363652076408No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCAATATCTCGTATGCCGT10800.1166673328227337No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA150.002220806470.03145667
GCGTTGT150.002220806470.03145666
CCGTTAG150.002225094269.99740632
AGGGGGG363350.066.7934670
ACTCGTA4100.060.60096438
TAACTCG4500.058.3248736
CCGATCA308.388703E-658.32171614
AAGGGGG419150.057.75633269
CTTAAAC10550.056.082451
TCGTAAG8950.055.91065240
TGCCGAC5400.055.72663545
TATCTCG430800.055.37613336
CCGTCTT415500.055.3656347
GCCGTCT418700.055.33230646
TATGCCG426450.055.32776643
ATATCTC431400.055.24317635
CGTCTTC418600.055.23983848
CTGCTTG412300.055.2356454
GTATGCC427550.055.23452442
ATGCCGT426250.055.19775844
Produced by FastQC (version 0.11.5)