FastQCFastQC Report
Thu 26 May 2016
SRR1781364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971000
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40074541.271369721936146No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG34480.35509783728115346No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11140.11472708547888774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT150.002220352270.03532452
CGTATCA7450.059.22144756
CGCCGTA7650.058.5916453
TCGCCGT8050.057.4202652
CCGTATC7500.056.49224555
GATCCGG252.3989071E-455.96188710
GTGGTCG9700.055.95316748
GCCGTAT8050.054.8102554
CTCGGTG9700.054.52327344
GATCTCG8300.054.43626441
GGTCGCC9400.054.38632650
TCGGTGG10750.053.75635545
GTCGCCG9450.053.730851
ATCTCGG8500.053.57023642
TCTCGGT9350.053.57023643
TGGTCGC9050.053.39434449
CGGTGGT10300.053.38744446
GTAGATC8550.052.84456338
CGATTTT200.006932252.53732736
CGACGTA200.006937857352.5264953
Produced by FastQC (version 0.11.5)