FastQCFastQC Report
Thu 26 May 2016
SRR1781364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971000
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT28847729.709268795056644No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG343803.540679711637487No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC157111.6180226570545828No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT66480.6846549948506694No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC60780.6259526261585994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60370.6217301750772399No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT44530.4585993820803296RNA PCR Primer, Index 20 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT32760.33738414006179196No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC25270.26024716786817714No Hit
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT21000.21627188465499483No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG20770.21390319258496396No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT17340.17857878475798145No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT16820.17322348094747683No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT13700.141091658084449No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC13120.13511843460350154No Hit
ATTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT12820.13202883625128733No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC12170.12533470648815653No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG12150.12512873326467558No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGCCATCTCGTATGCCGT11560.11905252317198764No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC11440.11781668383110196No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG10270.10576725025746653No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT9940.1023686920700309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG358200.066.80920470
GACGAAT352.9787407E-759.9905546
AAGGGGG419500.056.9410569
TCGTAAG6150.056.33549540
CGATCCC509.822543E-1055.9940715
CTGCTTG414750.054.98995654
CCGTCTT420500.054.94259647
TGCTTGA418450.054.9247255
TTGAAAA432350.054.88104258
ATCTCGT431650.054.8800937
TCTCGTA432200.054.8669338
CACGTAT3700.054.85905539
CTTGAAA428650.054.8292657
GCCGTCT424900.054.81838646
CATCTCG430900.054.8131836
TATGCCG430100.054.79025743
CGTCTTC422300.054.77470448
CTCGTAT432550.054.74162739
GCTTGAA428550.054.738456
GTCTTCT418750.054.73764849
Produced by FastQC (version 0.11.5)