FastQCFastQC Report
Thu 26 May 2016
SRR1781363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270139
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37668529.656990297912277No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG32720.257609600209111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG308.423238E-658.2833984
CGTATCA7950.056.37276556
CCGTATC7750.054.66725555
CGCCGTA7800.054.3168253
ATCTCGG8750.054.0431442
GATCTCG8900.053.13229841
GTTTCGC200.00696601852.4736521
CGATTTG200.00696601852.4736516
GCCGTAT8250.051.78054454
TGGTCGC9000.051.35206649
TCGCCGT8200.051.2402252
GTGGTCG9650.051.16057248
GCGTATC552.2791937E-950.92967655
TCTCGGT8900.050.77086343
GTAGATC9250.050.737238
TTGTGCG2500.050.362812
CTCGGTG10050.048.7874744
TAGATCT9550.048.77662339
GTCGCCG9100.048.48113351
GGTCGCC9250.048.0734850
Produced by FastQC (version 0.11.5)