FastQCFastQC Report
Thu 26 May 2016
SRR1781363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270139
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT27370721.549373729961836No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG329042.590582605525852No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC160791.2659244381914105No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT63060.4964810938015446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57560.4531787465781304No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC57280.4509742634467566No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT34080.2683170897043552No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT32970.2595778887192662No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT29450.2318643864962811No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT22050.17360304659568757No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC18430.14510222896864045No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG18350.14447237664539078No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT15770.12415963922058926No Hit
ATTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT13410.10557899568472426No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAAATATCTCGTATGCCGT12820.10093383480075803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG150.002224923370.00027529
AGGGGGG345400.067.3617670
AAGGGGG403450.057.582769
CCGTCTT405200.055.972447
GCCGTCT409250.055.9657546
TGCCGTC411350.055.9097345
TATGCCG413250.055.88131343
ATGCCGT412950.055.87954344
CGTCTTC410050.055.8138948
ATCTCGT417750.055.72772637
TCTCGTA417200.055.72569338
CTCGTAT416850.055.70093539
TATCTCG415300.055.68569636
GTATGCC415150.055.67614442
TGCTTGA410400.055.510755
GTCTTCT411300.055.5081349
TCGTATG421800.055.49527440
CTTCTGC411650.055.46943751
CTGCTTG408800.055.4581854
TTCTGCT408100.055.3945852
Produced by FastQC (version 0.11.5)