FastQCFastQC Report
Thu 26 May 2016
SRR1781362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285306
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20028970.2014678976257No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8790.3080902609829446No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6060.21240352463670586No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5740.2011874969331174No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5100.17875544152594056No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5070.17770393892872915No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4820.16894141728530068No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4400.15422038092434087No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3220.11286127876735856No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.1040987571239301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC150.002213232770.0792138
TGATCTC150.002213232770.0792140
AGATGTC301.0135591E-770.0792140
TTGATCT150.002213232770.0792139
GTGGATC150.002213232770.0792138
GGTAGAT401.3278623E-1070.079237
GAGATCT353.6670826E-970.079239
AGTGTGT550.070.03001433
CGCGGTA252.8282993E-670.03001453
TAGCATT207.908484E-570.0177258
GCAATAA150.002220952270.0177260
ATCAGGA252.8312497E-670.0177259
TATCAGT401.3460522E-1070.0177258
TATCAGG150.002220952270.0177258
AGCATTA252.8312497E-670.0177259
AATAAAG150.002220952270.0177262
GGTATCA301.01972546E-770.0177256
CAGTGAA150.002220952270.0177261
GTAGCAT207.908484E-570.0177257
CTTAGGG150.002222498470.005441
Produced by FastQC (version 0.11.5)