FastQCFastQC Report
Thu 26 May 2016
SRR1781362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285306
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT11273539.5137150988763No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3305811.58685761953832No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC80592.824686476975598No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC68772.4103944536743005No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT42821.5008447070864266RNA PCR Primer, Index 17 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAAAACAGATCTCGTATGCCGT27040.9477543409532222No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC20610.7223822842842422No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT15130.5303078098602904No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC13280.4654651496989197No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT12980.4549501237268056No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC12560.44022908736584576No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG11780.412890019838349No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATAAAGATCTCGTATGCCGT11700.4100860129124519No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT10280.3603148899777782RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT10110.3543563752602469No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTACAGATCTCGTATGCCGT9790.34314034755665845No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGACGT9550.33472832677896713No Hit
CTTATACACATCTCCGAGCCAACGAGACAAATACAGATCTCGTATGCCGT9470.33192431985307No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGA9190.32211029561243015No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG9070.3179042852235845No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCAGT7980.2796996908582364No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCG7820.2740916770064422No Hit
CTTATACACATCTCCGAGACCACGAGACAAATACAGATCTCGTATGCCGT7780.27268967354349366No Hit
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC7780.27268967354349366No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGAATGCCGT7350.2576181363167967No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT7260.25446362852516247No Hit
TCTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT7120.24955661640484253No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTAAGCCGT6640.23273257484945986No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT6200.2173105367570258No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5810.2036410029932774No Hit
CTTATACACATCTCCGAGCACACGAGACAAATACAGATCTCGTATGCCGT5590.19592998394706035No Hit
CTTATACACATCACCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT5450.1910229718267404No Hit
CTTATACACATCTCAGAGCCCACGAGACAAATACAGATCTCGTATGCCGT4980.1745494311370949No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT4840.169642419016775No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCAG4550.15947789391039796No Hit
TCTTATACACATCTCCGAGCCAACGAGACAAATACAGATCTCGTATGCCG4530.15877689217892368No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCACGTATGCCGT4150.14545785928091243No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACATATCTCGTATGCCGT3900.13669533763748395No Hit
CTTATACACAACTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3710.13003582118847834No Hit
ATTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3610.12653081253110696No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATACAGATCTCGTATGCCGT3570.1251288090681584No Hit
TCCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC3570.1251288090681584No Hit
CTTATACACATCTACGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3120.10935627010998716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGG150.002219570.0292868
AAGGGGA301.0185795E-770.0292869
CAAGGGG252.8284812E-670.0292868
AAGTGCG150.002219570.0292869
TGAAACA353.6925485E-970.0170159
ATGGGGT150.002221044470.01700670
AGGGGAG150.002221044470.01700670
AGTGGGG600.070.01700670
AGGGTGG252.831428E-670.01700670
AGGGAGT150.002221044470.01700670
ATGTGGG150.002221044470.01700670
ATGAAAC207.9157675E-570.0047358
ATTTAAA207.9157675E-570.0047357
CGTCAAA150.002224135269.9924748
AACGCCA150.002224135269.9924729
GAGAAAT150.002224135269.9924726
TTCATAA150.002224135269.9924752
TCCCAAG150.002224135269.9924718
TAAACGA301.0222902E-769.9924711
AGCTTCA455.456968E-1269.9924755
Produced by FastQC (version 0.11.5)