FastQCFastQC Report
Thu 26 May 2016
SRR1781361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654413
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16525425.252248962046902No Hit
CTTATACACATCTGACGCTGCCGACGACCTTCACCGTGTAGATCTCGGTG10820.16533901374208643No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA150.002217243270.0574940
TTATGCG207.956952E-569.945052
TTGTACG207.956952E-569.945052
CGTTAAG150.00223141369.945052
ACGCGTG150.002232089669.9397056
GTACGCG308.41896E-658.283094
TCATCGA200.00692817452.54311437
CACGGTG200.00693027152.53909344
ACGTATC200.006934467752.5310555
CGACGTA200.00693656752.5270353
CGTATCT200.00694496852.5109670
GATCGTC200.00694706952.50694332
CTCGACT200.006959687552.48285328
CGGTCGC200.006959687552.48285328
CGTGTAC200.00696389752.474831
CGCTGCG800.052.47081816
GCACTCG200.006966002852.47081820
TTGTGCG407.519993E-752.458792
ACACGGC200.006974430352.454786
TACGAGA200.006974430352.454785
Produced by FastQC (version 0.11.5)