FastQCFastQC Report
Thu 26 May 2016
SRR1781361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654413
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT7837411.976229078578818No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG250483.827552325519206No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC62880.9608611075880217No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC53010.8100389203759705No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT36000.5501113211381803RNA PCR Primer, Index 43 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT35290.5392619034157329No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21950.33541509719397383No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT20350.31096570514338806No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT18180.2778062171747811No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC13940.21301532824072872No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGACGT12630.19299738849931158No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG12280.18764908398824595No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC10670.163046883237344No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGA10220.15617049172311676No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGAATCTCGTATGCCGT9880.15097499591236727No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC9870.15082218721205112No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTAGTATGCCGT9480.1448626478997208No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGAATGCCGT7780.11888516884597342No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG7760.1185795514453411No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAACAGCATCTCGTATGCCGT7500.1146065252371209No Hit
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT7260.11093911642953302No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT6920.10574362061878356RNA PCR Primer, Index 43 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG6890.10528519451783508No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTC150.002222847770.0129257
CGTCGAT150.002222847770.012921
CAGCGTA150.00222554469.99151630
TTGGCGA150.00222554469.99151623
GCGACTA150.00222554469.99151626
CGCGCAA150.00222554469.99151620
ACCGTCA150.00222554469.99151646
GGCGACT150.00222554469.99151625
AGGGGGG113850.063.22730370
GAATCTC3900.058.32626335
ATACCGT252.3917052E-455.99321744
CCAGAAT3150.055.5488332
CCCGCAT1500.053.66016832
ACTCGAA1050.053.32687438
GCTATCC200.006941437752.51771561
GTCTTGC200.006945635752.5096957
AGCGTAA200.00695403752.49363731
CGACATT200.00695403752.49363741
TAGCCCG200.00695403752.49363731
ACAGCGT200.00695403752.49363729
Produced by FastQC (version 0.11.5)