FastQCFastQC Report
Thu 26 May 2016
SRR1781360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228934
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16548472.28458857137865No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5190.22670289253671366No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4060.17734368857399951No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.16773393205028522No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3410.148951226117571No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3090.1349733984467139No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2930.12798448461128534No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2920.1275476774965711No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2720.11881153520228538No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2690.11750111385814253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA353.6707206E-970.0614956
TGATCTT150.002214891470.06148538
GGTAGCA207.880709E-570.06148556
ATTTTTA150.002214891470.06148556
TAGATGT150.002214891470.06148539
GGTGAGT150.002216814870.04616558
ATGGCCA150.002216814870.04616548
TTTTTAT150.002216814870.04616557
TTTTATT150.002216814870.04616558
TAGCAGG150.002216814870.04616558
GGGTGAT150.002216814870.04616535
GATCATT207.889257E-570.0461658
GTAGCAT207.889257E-570.0461657
GTAGCAG353.6761776E-970.0461657
GCATGAA150.002218739470.03084660
AGCATTA150.002218739470.03084659
TCACAGC150.002218739470.03084669
GCATTAA150.002218739470.03084660
CACAGCG150.002218739470.03084670
CGTCACA150.00222066570.01552667
Produced by FastQC (version 0.11.5)