FastQCFastQC Report
Thu 26 May 2016
SRR1781360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228934
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT8927038.993771130544175No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG2766412.083832021455967No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC71103.1056985856185624No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC56132.451798334891279No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT38071.6629246857172808RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT25951.1335144626835683No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT18200.7949889487799977No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC16740.7312151100317122No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT12530.5473193147369985No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC10990.4800510190709986No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC10170.4442328356644273No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT10140.44292241432028445No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT9910.43287585068185586No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG9170.4005521241929989No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACGT8590.37521731153957033No Hit
CTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCGT7850.3428935850507133No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG7640.33372063564171334No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT7140.31188027990599915No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCAGT7090.3096962443324277No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGA6700.29266076685857056No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC6410.27999336053185636No Hit
CTTATACACATCTCCGAGACCACGAGACGACTTAGTATCTCGTATGCCGT6410.27999336053185636No Hit
TCTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5640.24635921269885644No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGAATGCCGT5440.23762307040457076No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG5380.23500222771628507No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTAAGCCGT5320.23238138502799932No Hit
CTTATACACATCTCCGAGCACACGAGACGACTTAGTATCTCGTATGCCGT4400.19219513047428516No Hit
CTTATACACATCACCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT4300.1878270593271423No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTAAGAATCTCGTATGCCGT4290.18739025221242805No Hit
TCTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCG4050.1769068814592852No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCAG4010.17515965300042807No Hit
CTTATACACATCTCAGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT3950.17253881031214238No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTAGTATCTCGTATGCCGT3830.16729712493557095No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTAACTCGTATGCCGT3610.15768736841185668No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT3250.14196231228214246No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCG3020.13191574864371391No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC2910.12711087038185678No Hit
GCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG2840.1240532205788568No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGAATCTCGTATGCCGT2710.11837472808757109No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTTAGTATCTCGTATGCCGT2680.11706430674342824No Hit
CTTATACACAACTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT2570.11225942848157111No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATATCGTATGCCGT2470.10789135733442826No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACG2420.10570732176085684No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCG2350.10264967195785683No Hit
ATTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT2290.10002882926957114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTG150.002220971770.01311569
AAGGTGT150.002220971770.01311569
AAAATGT150.002220971770.01311568
AATTGGG150.002220971770.01311569
AGTGGGG252.8305749E-670.0131170
AGGGTGG401.3460522E-1070.0131170
AGGGAGG252.8305749E-670.0131170
TCCTTAT600.069.997821
GTCATTA150.002222897369.9978244
CCTATGC207.916304E-569.9978241
TATACTG150.002222897369.9978250
TAAATCG401.3460522E-1069.9978236
ATAAGTA301.02083504E-769.9978231
ACTGATA150.002222897369.9978253
TATACCA150.002222897369.9978216
GAAACGA150.002222897369.9978224
TTCGTAT150.002222897369.9978232
ACCACTT150.002222897369.9978227
TCCTAGC401.3460522E-1069.9978213
AACGACT150.002222897369.9978226
Produced by FastQC (version 0.11.5)