FastQCFastQC Report
Thu 26 May 2016
SRR1781359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258811
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18960473.25963734153494No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6930.26776296216157736No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4740.18314522953042955No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4440.17155375930698463No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4270.1649852595136992No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4180.1615078184466657No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3950.15262102460869129No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3230.1248014960724235No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.11475555521210459No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850.11011896712272663No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2750.10625514371491165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATG150.0022172870.04507438
GGATGTT150.0022172870.04507434
AGCTCGG150.0022172870.04507442
GCTACTA150.0022172870.04507442
AGATGGC150.0022172870.04507440
GGTAGAT252.8239465E-670.04507437
ATGTTGG150.0022172870.04507436
AGGTTGG207.891813E-570.04507436
GTAGGTT150.0022172870.04507434
GTAGGGC207.891813E-570.04507438
CAGTGTA252.8239465E-670.04507434
TACTAGG150.0022172870.04507444
TAGGTTG207.891813E-570.04507435
TGATGGC150.002218982270.0315347
GATATAC150.002220685470.0179952
GGATATA150.002220685470.0179951
ATACGCT150.002220685470.0179955
GATTCAG150.002220685470.0179950
ATATACG150.002220685470.0179953
CCAGTGT207.906953E-570.0179833
Produced by FastQC (version 0.11.5)