FastQCFastQC Report
Thu 26 May 2016
SRR1781359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258811
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT10102639.034662359791504No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3042011.753750806573136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC79673.078308109006186No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC61712.384365424962618No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT39851.5397336280142653RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT22880.8840427957080649No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT19560.755763858568608No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC18960.7325809181217182No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT15520.5996653928928832No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC12560.48529622002156014No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT12450.48104601427296373No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC11230.433907368697621No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG10790.41690654570323515No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAAATCTCGTATGCCGT10780.4165201633624537No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC10180.3933372229155639No Hit
CATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT10180.3933372229155639No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGACGT9540.3686087531055481No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT9370.36204025331226264No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGA8930.34503943031787676No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT8830.34117560691006177No Hit
CTTATACACATCTCCGAGCCAACGAGACTCTTATTAATCTCGTATGCCGT8800.3400164598877173No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG8350.3226292545525499No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCAGT7920.3060148138989456No Hit
CTTATACACATCTCCGAGACCACGAGACTCTTATTAATCTCGTATGCCGT6860.2650582857761069No Hit
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC6770.2615808447090734No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG6530.2523076685303175No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAAACTCGTATGCCGT6430.2484438451225025No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGAATGCCGT6270.24226172766999857No Hit
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT6020.23260216915046117No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATAATAATCTCGTATGCCGT5170.19975967018403393No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTATTAATCTCGTATGCCGT4960.19164564102762247No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC4950.191259258686841RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTAAGCCGT4690.18121331782652206No Hit
CTTATACACATCTCCGAGCACACGAGACTCTTATTAATCTCGTATGCCGT4400.17000822994385864No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCAG4320.16691717121760666No Hit
TCTTATACACATCTCCGAGCCAACGAGACTCTTATTAATCTCGTATGCCG3860.1491435835416578No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAAATAATCTCGTATGCCGT3680.14218870140759088No Hit
TCCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC3600.1390976426813389No Hit
CTTATACACATCTCAGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT3590.1387112603405574No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCG3490.13484743693274243No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGT3310.12789255479867548No Hit
CTTATACACATCACCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT3250.1255742607539865No Hit
GCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG2870.11089173180428961No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT2860.11050534946350811No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT2840.10973258478194513No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGACG2820.10895982010038212No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCG2620.10123217328475219No Hit
CTCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG2600.10045940860318921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG207.911838E-570.0092870
TATACGC252.8325412E-670.009283
TTATACG252.8325412E-670.009282
AGGTGGG252.8325412E-670.0092870
ATCGGGG207.911838E-570.0092870
CTTTACA1100.070.009271
TATATCC150.002221783470.009273
GGGTACT150.002221783470.009271
AGAGGCG150.002221783470.0092770
AAGAGGC150.002221783470.0092769
CTTAGAC150.002221783470.009272
TGATACA150.002221783470.009272
GTATATC150.002221783470.009272
TACGCAT301.0202166E-770.009275
AATGGTG150.002221783470.0092769
CGTATAT150.002221783470.009271
ATATCCA150.002221783470.009274
TAGACAC150.002221783470.009274
TCTTAGA150.002221783470.009271
TTATGCC550.069.9957542
Produced by FastQC (version 0.11.5)